Graphical Abstract: After modification of enotannins, flavonols monomer units could be obtained with polyfunctional properties. Moreover, polyphenols are known to generate H2O2 and other ROS under alkaline conditions. ROS generation and catalytic influence of ferrous ions enhance the chemiluminescence light. Therefore in this work, Tox/Tris-Edta/Fe2+ ions have been used for screening luminescent properties of oxidized tannins.